RBS

Part:BBa_K2182004:Design

Designed by: Vasileios Kreouzis, David Rosenberg   Group: iGEM16_Northeastern   (2016-10-14)


RBS for BBa_K2182003

This is the RBS submitted with BBa_K2182003 (Nadh Oxidase expression vector).


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The RBS is flanked by EcoRII and MspJI cut sites and has been modified to reduce the formation of primer homodimers during PCR.

Source

Ribosome binding site for FNR protein expression in E. coli submitted by TU Eindhoven 2013 (BBa_K1123010).

References